Laboratory for Bio-Organic Chemistry and Bio-Crystallography
In the laboratory GM Escherichia coli is used to produce proteins from Erwinia amylovora to be studied by protein X-ray crystallography. The crude extract from the bacterial cultures is loaded onto chromatography columns for the purification of the proteins under investigation. The pure proteins are then crystallized to solve their structure at the atomic level.
Purification and crystallization of proteins mainly involved in Erwinia amylvora pathogenicity.